Comparative Studies on Heterotrophic Growth of Thermophilic Chlorella

Author: Rong-Fong Lin,Tan-Chi Huang(Institute of Botany, Academia Sinica)

Vol.&No.：Vol. 47, No. 1
Date：April 2002
Pages：31-40
DOI：10.6301/JNTNU.2002.47(1).02

Abstract：

The genus Chlorella comprises species which are widely diversified in their physiological and ecological properties. Among them the species which can grow at 38 °C has been grouped as the thermophilic Chlorella. The thermophilic green microalgae have attained great importance not only in basic research but also in various fields of biotechnology. Properties of 5 thermophilic isolates, including SAG 211-31 from Germany, SAG 211-32 and SAG 211-34 from Japan, W-87 and T-89 from Taiwan, were examined. They are able to use urea as sole nitrogen source and no growth factor is needed for their cultivation. They grow rapidly by multiple fission (the optical density of culture was double within 4 h.) in the presence of glucose or acetate. The cells contained chlorophyll even after 10 days (more than 30 generations) of dark heterotrophic growth. Random amplified polymorphic DNA (RAPD) reaction was proved to be a feasible method for identification or differentiation among thermophilic isolates.

Keywords：Heterotrophic growth, Thermophilic Chlorella, Dark growth

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