Salt-Coated Sillca Gel Thin Layer Chromatography of Vitamin B Complex

Author: Bao-tyan Huang

Abstract：

This paper deals with the experiment on the acceleration of the speed of development by way of the thin-layer chromatography of Vitamin B complex, by using silica gel G or silica gel GF₂₅₄ and 5%, or 10%, or 20% of NH₄Cl, or NaCl, or A1₂(S0₄)₃, or Na₂S0₄ (by weight of salt solution) as coating agent instead of water.
The result of the experiment is that a mixture of acetone and alcohol (1:1 v/v) as developing solvent, and silica gel G which contains 5% NH₄C1, or NaCl, or A1₂(S0₄)₃, or 10% NH₄C1, or NaCl, has been proved experimentally to be suitable for the separation of Vitamin B complex.
The greatest advantage of this salt-coated silica gel G, or silica gel GF₂₅₄ is that it enables the process of experiment to accelerate the speed of development.

《Full Text》