鹽類與矽膠混合的薄層之色層分析法分離綜合維生素B

作者:黃寶鈿

摘要：

以各種鹽類與矽膠(Silica Gel)混合之薄層色層分析法，分離綜合維生素B(Vitamin B Complex)，所試用之鹽類為氯化鈉、氯化銨、硫酸鋁及硫酸鈉的水溶液，以其與矽膠混合作薄層。
據實驗結果，含鹽類薄層，可縮短展開所需的時間，薄層不易脫落，並且分離效果較佳等優點。以無水酒精及丙酮(1:1 v/v)混合液作展開劑時，含5%氯化銨（氯化鈉或硫酸鋁）及10%氯化銨（或氯化鈉）與矽膠之混合薄層，最適宜分離綜合維生素B。

《詳全文》

Salt-Coated Sillca Gel Thin Layer Chromatography of Vitamin B Complex

Author: Bao-tyan Huang

Abstract：

This paper deals with the experiment on the acceleration of the speed of development by way of the thin-layer chromatography of Vitamin B complex, by using silica gel G or silica gel GF₂₅₄ and 5%, or 10%, or 20% of NH₄Cl, or NaCl, or A1₂(S0₄)₃, or Na₂S0₄ (by weight of salt solution) as coating agent instead of water.
The result of the experiment is that a mixture of acetone and alcohol (1:1 v/v) as developing solvent, and silica gel G which contains 5% NH₄C1, or NaCl, or A1₂(S0₄)₃, or 10% NH₄C1, or NaCl, has been proved experimentally to be suitable for the separation of Vitamin B complex.
The greatest advantage of this salt-coated silica gel G, or silica gel GF₂₅₄ is that it enables the process of experiment to accelerate the speed of development.